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101.
《Molecular membrane biology》2013,30(5-8):198-207
AbstractThe PapC usher, a β-barrel pore in the outer membrane of uropathogenic Escherichia coli, is used for assembly of the P pilus, a key virulence factor in bacterial colonization of human kidney cells. Each PapC protein is composed of a 24-stranded β-barrel channel, flanked by N- and C-terminal globular domains protruding into the periplasm, and occluded by a plug domain (PD). The PD is displaced from the channel towards the periplasm during pilus biogenesis, but the molecular mechanism for PD displacement remains unclear. Two structural features within the β-barrel, an α-helix and β5-6 hairpin loop, may play roles in controlling plug stabilization. Here we have tested clusters of residues at the interface of the plug, barrel, α-helix and hairpin, which participate in electrostatic networks. To assess the roles of these residues in plug stabilization, we used patch-clamp electrophysiology to compare the activity of wild-type and mutant PapC channels containing alanine substitutions at these sites. Mutations interrupting each of two salt bridge networks were relatively ineffective in disrupting plug stabilization. However, mutation of two pairs of arginines located at the inner and the outer surfaces of the PD resulted in an enhanced propensity for plug displacement. One arginine pair involved in a repulsive interaction between the linkers that tether the plug to the β-barrel was particularly sensitive to mutation. These results suggest that plug displacement, which is necessary for pilus assembly and translocation, may require a weakening of key electrostatic interactions between the plug linkers, and the plug and the α-helix. 相似文献
102.
103.
Emily J Fleming Ivona Cetini? Clara S Chan D Whitney King David Emerson 《The ISME journal》2014,8(4):804-815
Despite over 125 years of study, the factors that dictate species dominance in neutrophilic iron-oxidizing bacterial (FeOB) communities remain unknown. In a freshwater wetland, we documented a clear ecological succession coupled with niche separation between the helical stalk-forming Gallionellales (for example, Gallionella ferruginea) and tubular sheath-forming Leptothrix ochracea. Changes in the iron-seep community were documented using microscopy and cultivation-independent methods. Quantification of Fe-oxyhydroxide morphotypes by light microscopy was coupled with species-specific fluorescent in situ hybridization (FISH) probes using a protocol that minimized background fluorescence caused by the Fe-oxyhydroxides. Together with scanning electron microscopy, these techniques all indicated that Gallionellales dominated during early spring, with L. ochracea becoming more abundant for the remainder of the year. Analysis of tagged pyrosequencing reads of the small subunit ribosomal RNA gene (SSU rRNA) collected during seasonal progression supported a clear Gallionellales to L. ochracea transition, and community structure grouped according to observed dominant FeOB forms. Axis of redundancy analysis of physicochemical parameters collected from iron mats during the season, plotted with FeOB abundance, corroborated several field and microscopy-based observations and uncovered several unanticipated relationships. On the basis of these relationships, we conclude that the ecological niche of the stalk-forming Gallionellales is in waters with low organic carbon and steep redoxclines, and the sheath-forming L. ochracea is abundant in waters that contain high concentrations of complex organic carbon, high Fe and Mn content and gentle redoxclines. Finally, these findings identify a largely unexplored relationship between FeOB and organic carbon. 相似文献
104.
《Current biology : CB》2022,32(4):763-774.e4
105.
《Harmful algae》2014
Populations of Noctiluca scintillans (hereafter Noctiluca) were compared from two regions: the northeastern-central Black Sea and the northern Adriatic Sea. In both seas samples were collected in near-shore waters 2–3 times per month during 2004–2012. For analysis of feeding activities and seasonal dynamics additional cruise data on the open waters of the Black Sea were used. Comparison between the two populations shows similarity in size structure with two classes 401–500 μm and 501–600 μm being the most numerous. Seasonal changes in cell abundance in both seas demonstrated a regular annual maximum with the peak period of high abundances in May–June with additional sporadic peaks in other seasons. In spring the average number of food vacuoles in the cell (1.78) and the proportion of feeding cells in populations (79%) in the Adriatic Sea were similar to those in the Black Sea (1.58 and 76%). In September–October, these parameters were lower both in the Adriatic Sea (0.69 and 49%) and in the Black Sea (1.46 and 65%) demonstrating that Noctiluca was better provided with food in spring. Among biotic parameters (wet phytoplankton biomass, chlorophyll biomass and zooplankton species) only the concentration of the eggs of Calanus euxinus was significantly positively correlated with abundance of Noctiluca. The possible effect of a high concentration of copepod eggs on the growth of Noctiluca in the peak period is discussed. An obvious negative relationship was observed between Noctiluca cell numbers in the peak period and wind velocity in both seas. The most significant negative correlation was observed between the number of windy hours per month (velocity more than 5–6 m s−1) and cell concentrations in the Black Sea (r = −0.92) and in the northern Adriatic Sea (r = −0.67). On this basis, a new hypothesis has been proposed and discussed: in connection with features of the food behavior of Noctiluca, its outbursts during the peak period are controlled by the wind. An evident positive relationship was observed between the number of Noctiluca in the peak period and its quantity in the preceding months in both seas. Thus, we suggest that abundance data during early spring and weather forecasts (winds) may be used for medium-term prediction of Noctiluca outbursts and red tides. 相似文献
106.
107.
冰川景观变化是全球环境和气候变化的共同"指示器"。随着全球气候变化加剧,干旱和半干旱地区的冰川景观将进一步变化。科学评估冰川景观格局时空演变特征,为应对冰川景观变化及其影响提供科学参考。基于1991-2017年Landsat TM/ETM+/OLI遥感影像,采用监督分类方法获取叶尔羌河流域冰川景观时空格局及变化数据,利用景观格局指数、质心迁移模型、分形维数等方法分析近26年叶尔羌河流域冰川景观格局时空演变特征。结果表明:(1)1991-2017年叶尔羌河流域冰川斑块面积呈消减趋势,但有所减缓,共减少799.50 km2(-13.09%)。冰川斑块面积消减主要集中在海拔5400-5800 m之间,相较之下,海拔4400 m以下的区域冰川斑块面积消减率最高,达63.68%;不同坡度冰川景观消减率存在差异,陡坡冰川斑块面积消减率最高(15.98%),急陡坡消减率最低(2.87%);阴阳两坡冰川景观均呈消减趋势,阳坡冰川斑块面积消减速率显著高于阴坡。(2)近26年来,叶尔羌河流域冰川斑块数量、最大斑块指数均减小,而平均形状指数、平均周长面积比、分裂指数均增加,表明冰川景观不断消减,破碎化程度增加。(3)研究期间,叶尔羌河流域冰川景观质心发生迁移,整体呈现东北偏移趋势。(4)通过分形理论对叶尔羌河流域冰川景观空间结构特征进行分析表明,该流域冰川景观消减率略微降低,但仍然处于持续消融状态。 相似文献
108.
B.I. Van Tussenbroek 《欧洲藻类学杂志》2013,48(3):161-165
Fluctuations in plant and frond characteristics are described for Macrocystis pyrifera (L.) C. Agardh (Laminariales, Phaeophyta) forming a fringing zone in the Falkland Islands. Giant kelp plants were sampled along a transect in the austral autumn (May 1986) and late spring (December 1986) which, according to previous frond weight analysis, were the times when extremes in population parameters were expected. Plant density and holdfast wet weights were similar for both seasons, but plants had more fronds and the fronds weighed more in spring than in autumn. Consequently, in autumn the frond biomass (1·1 wet kg m?2) and productivity (34·1 wet g m?2 d?1) were lower than in spring, when a biomass of 5·0 wet kg m?2 and a productivity of 72·4 wet g m?2 d?1 were recorded. Production of new fronds and loss of old fronds were determined at monthly intervals between April 1986 and March 1987. New frond production rates followed fluctuations in the quantity of light and varied between 0·08 and 0·48 fronds per plant per day. Frond loss rates did not show a seasonal pattern and fluctuated between 0·05 and 0·42 fronds per plant per day. It is suggested that the Falkland Islands Macrocystis population is more stable than most other giant kelp beds at high latitudes, because of the absence of winter storms. 相似文献
109.
A. S. Bains E. S. Boek P. V. Coveney S. J. Williams M. V. Akbar 《Molecular simulation》2013,39(2):101-145
Abstract It is well known that the sodium smectite class of clays swells macroscopically in contact with water, whereas under normal conditions the potassium form does not. In recent work using molecular simulation methods, we have provided a quantitative explanation both for the swelling behaviour of sodium smectite clays and the lack of swelling of potassium smectites [1]. In the present paper, we apply similar modelling methods to study the mechanism of inhibition of clay-swelling by a range of organic molecules. Experimentally, it is known that polyalkylene glycols (polyethers) of intermediate to high relative molecular mass are effective inhibitors of smectite clay swelling. We use a range of atomistic simulation techniques, including Monte Carlo and molecular dynamics, to investigate the interactions between a selection of these compounds, water, and a model smectite clay mineral. These interactions occur by means of organised intercalation of water and organic molecules within the galleries between individual clay layers. The atomic interaction potentials deployed in this work are not as highly optimised as those used in our clay-cation-water work [1]. Nevertheless, our simulations yield trends and results that are in qualitative and sometimes semi-quantitative agreement with experimental findings on similiar (but not identical) systems. The internal energy of adsorption of simple polyethers per unit mass on the model clay is not significantly different from that for water adsorption; our Monte Carlo studies indicate that entropy is the driving force for the sorption of the simpler organic molecules inside the clay layers: a single long chain polyethylene glycol can displace a large number of water molecules, each of whose translational entropy is greatly enhanced when outside the clay. Hydrophobically modified polyalkylene glycols also enjoy significant van der Waals interactions within the layers which they form within the clay galleries. In conjunction with experimental studies, our work furnishes valuable insights into the relative effectiveness of the compounds considered and reveals the generic features that high performance clay-swelling inhibitors should possess. For optimal inhibitory activity, these compounds should be reasonably long chain linear organic molecules with localised hydrophobic and hydrophilic regions along the chain. On intercalation of these molecules within the clay layers, the hydrophobic regions provide an effective seal against ingress of water, while the hydrophilic ones enhance the binding of the sodium cations to the clay surface, preventing their hydration and the ensuing clay swelling. 相似文献
110.
We carried out NPT molecular dynamics simulations in an explicit solvent to better understand the mechanism of cyclic adenosine monophosphates (cAMP) hydrolysis by phosphodiesterase 4 (PDE4) enzyme on atomic details and to obtain information on the dynamics characteristic of the catalytic domains of PDE4. In analyzing the water hydrogen-bond network around the active site, we also showed the importance of water in drug–protein interactions. In addition, we reported the characteristics of the hydration pattern and the dynamic distance distribution around the interesting residues. The results indicated that Asp318 plays the role of a general base that can activate water molecule for nucleophilic attack on cAMP. As expected, His160 plays the role of a proton donor for cAMP. 相似文献